IL18 Antibody

• Catalog Number: orb1274549
• Category: Antibodies
• Description: IL18 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 23kDa
• Target: IL18
• UniProt ID: Q14116
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: IL18, interleukin 18 (interferon-gamma-inducing fa
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IL18 antibody (orb1274549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug IL18 antibody (orb1274549). Western blot was performed from the immunoprecipitate using IL18 antibody (orb1274549) at a dilution of 1:1000.