Il17f Antibody

• Catalog Number: orb1671705
• Category: Antibodies
• Description: Il17f Antibody
• Clonality: Recombinant
• Clone Number: 8F5.1A9
• Tested applications: FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising mice with murine IL-17F coupled to ovalbumin to generate a highly immunogenic complex.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Il17f
• UniProt ID: Q7TNI7
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-17F, IL17F
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of 8F5.1A9 (orb1671705 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.