IL16 Antibody

• Catalog Number: orb1671805
• Category: Antibodies
• Description: IL16 Antibody
• Clonality: Recombinant
• Clone Number: 14.1
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: The antibody was raised against IL-16 using a synthetic peptide corresponding to the predicted amino acid sequence of a hydrophilic region of IL-16, conjugated to keyhole limpet hemocyanin.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: IL16
• UniProt ID: Q14005
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: LCF, interleukin 16, NPRprIL-16, IL16
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-16 antibody 14.1. Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of 14.1 (orb1671805 - pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with orb1671805. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.