IL12 p40 antibody

• Catalog Number: orb19546
• Category: Antibodies
• Description: Goat polyclonal antibody to IL12 p40
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 37.2
• Target: IL12B / IL12p40
• Entrez: 3593
• Protein Sequence: QGKSKREKKDRVFTD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-CLMF antibody, anti-CLMF p40 antibody, anti-C
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Colon shows cytoplasm staining of individual cells between columns. In paraffin embedded Human Kidney shows cytoplasm staining of epithelial cells of some DCT. Recommended concentration, 4-6ug/ml.WB: Approx 37kDa band observed in Human Liver lysates (calculated MW of 37.2kDa according to NP_002178.2). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19546 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19546 (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 9, HRP-staining.

Western blot analysis of Mouse Liver (lane 1) and Testes (lane 2) and Rat Brain (lane 3) lysate using IL12 p40 antibody