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Catalog Number | orb1272491 |
---|---|
Category | Antibodies |
Description | IL11 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-11 (human Interleukin-11). |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-11 (1.50 ng/mL), a concentration of 0.02 - 0.05 μg/mL of this antibody is required. ELISA:To detect hIL-11 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-11.Sandwich:To detect hIL-11 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-11as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-11. Western Blot:To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | IL11 |
UniProt ID | P20809 |
NCBI | P20809 |
Storage | IL-11 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | AGIF, IL-11Interleukin-11, Adipogenesis inhibitory Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect hIL-11 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human IL-11 (orb1272490) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-11.
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 mg/ml overnight at 4°C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P | |
Bovine, Canine, Guinea pig, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P | |
Bovine, Canine, Guinea pig, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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