IL-9R Antibody [AH9R2], Rabbit IgG

• Catalog Number: orb1671707
• Category: Antibodies
• Description: IL-9R Antibody [AH9R2], Rabbit IgG
• Clonality: Recombinant
• Clone Number: AH9R2
• Tested applications: Blocking, IP
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising DBA/2 mice with P815 mastocytoma cells transfected with human IL-9R cDNA.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: IL9R
• UniProt ID: Q01113
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-9 receptor, IL9R, CD129
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-9R antibody AH9R2. Jurkat cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r1 version of HB-8592 (orb1671707 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody (AH9R2). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of AH9R2 (orb1671707) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671707- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.