IL-9 Antibody [RM9A4], Rabbit IgG

• Catalog Number: orb1671710
• Category: Antibodies
• Description: IL-9 Antibody [RM9A4], Rabbit IgG
• Clonality: Recombinant
• Clone Number: RM9A4
• Tested applications: ELISA, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising rats with recombinant full-length murine IL-9.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Il9
• UniProt ID: P15247
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-9, IL9, Cytokine P40, T-cell growth fa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-9 antibody RM9A4. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of RM9A4 (orb1671710- pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with orb1671710. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.