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IL-7 Antibody Peroxidase Conjugated
Catalog Number: orb345167
| Catalog Number | orb345167 |
|---|---|
| Category | Antibodies |
| Description | IL-7 antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, IHC, WB |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein. |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
| Conjugation | HRP |
| UniProt ID | P13232 |
| NCBI | NP_000871.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-IL-7 peroxidase conjugated antibody, r Read more... |
| Note | For research use only |
| Application notes | This purified antibody has been tested in western blotting and ELISA. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
Biorbyt's anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n orb347654) secondary antibody diluted 1:40000 in blocking buffer (p/n orb348637) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate.
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