IL-6R alpha Rabbit Polyclonal Antibody

• Catalog Number: orb1151987
• Category: Antibodies
• Description: IL-6R alpha Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human IL-6R alpha (20-365/468aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: IL6R
• UniProt ID: P08887
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IL6R; CD 126; CD126; CD126 antigen; gp80; IL 6R 1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (orb1151987) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (orb1151987) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant Human IL-6R alpha Protein at 50 ng, Lane 2: Recombinant Human IL-6R alpha Protein at 500 ng, Primary: Rabbit Anti-IL-6R alpha Antibody at 1/1000, dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42kD, Observed band size: 58-75kD.