IL-5 Antibody [2E3], Rabbit IgG

• Catalog Number: orb1671743
• Category: Antibodies
• Description: IL-5 Antibody [2E3], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 2E3
• Tested applications: ELISA, NeA
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising CAF1 mice with human recombinant IL-5.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: C9JQP9
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: interleukin 5, IL5, IL 5, Eosinophil differentiati
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-5 antibody 2E3. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of 2E3 (orb1671743 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.