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    IL-32A antibody (Biotin)

    Catalog Number: orb345175

    DispatchUsually dispatched within 5-10 working days
    $ 753.00
    Catalog Numberorb345175
    CategoryAntibodies
    DescriptionIL-32A antibody (Biotin)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityHuman
    IsotypeIgG
    ImmunogenThis purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
    Form/AppearanceLyophilized
    PurityThis purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-32A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
    ConjugationBiotin
    UniProt IDP24001
    NCBI61658634
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesrabbit anti-IL-32A biotin conjugated antibody, rab
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    NoteFor research use only
    Application notesThis purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to native human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
    Expiration Date12 months from date of receipt.
    IL-32A antibody (Biotin)

    Western blot analysis of higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5

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