You have no items in your shopping cart.
Cart summary
Item 1 of 1
IL-3 Antibody Biotin Conjugated
Catalog Number: orb345180
| Catalog Number | orb345180 |
|---|---|
| Category | Antibodies |
| Description | IL-3 antibody (Biotin) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein. |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
| Form/Appearance | Lyophilized |
| Purity | Biotin Conjugated Anti-Human IL-3 Antibody Biotin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
| Conjugation | Biotin |
| UniProt ID | P08700 |
| NCBI | NP_000579 |
| Storage | Store Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-IL-3 biotin conjugated antibody, rabbi Read more... |
| Note | For research use only |
| Application notes | This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Human IL-3 Biotin Conjugated Antibody. Lane 1: Human IL-3. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human IL-3 primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for Human IL-3. Other band(s): None.
