IL-25 Antibody / IL-17E

• Catalog Number: orb2635111
• Category: Antibodies
• Description: Cytokines are small, soluble proteins with pleiotropic effects on a variety of cell types. Cytokines have a regulatory function over the immune system and mediate aspects of inflammatory response. They exert their biological effects through the binding of membrane-bound receptors which, in turn, initiate signal transduction cascades and elicit physiological changes in their target cell. Interleukin-17 (IL-17) and its cognate receptor, IL-17R, are an example of such a cytokine receptor pair. Originally identified as a rodent cDNA termed CTLA8, IL-17 is capable of inducing the secretion of IL-6 and IL-8 and augmenting the expression of ICAM-1 in human fibroblast cultures. The IL-17 protein exhibits a striking degree of homology with the HSV-13 protein which mimics its function. The IL-17 receptor is a type I transmembrane protein 864 amino acids in length that is highly expressed in spleen and kidney.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant protein fragment was used as the immunogen for the IL-25 antibody.
• UniProt ID: Q9H293
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the IL-25 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: IL25/625
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carcinoma tissue with IL-25 antibody (clone IL25/625). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human adrenal gland tissue with IL-25 antibody (clone IL25/625). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free IL-25 antibody (clone IL25/625) as confirmation of integrity and purity.