IL-1F5 antibody

• Catalog Number: orb345076
• Category: Antibodies
• Description: IL-1F5 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 8,000, WB: 1 µg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-1F5 protein. A BLAST analysis was used to suggest cross-reactivity with IL-1F5 from dog, panda, and ferret based on 90-92% homology with the immunizing sequence.
• Conjugation: Unconjugated
• UniProt ID: Q0VC52
• NCBI: 115494990
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-IL-1F5 antibody, rabbit anti-Interleuk
• Note: For research use only
• Application notes: This protein-A purified Interleukin-1F5 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kDa in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of shows detection of recombinant bovine using IL-1F5 antibody