IL-17F Antibody

SKU: orb345105

Description

IL-17F antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 5µg/mL, WB: 0.5µg/mL
• Reactivity: Human
• Application Notes:
  Anti-Human IL-17F antibody has been tested for use in IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4H1629.1
• Immunogen: Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development.
• Purity: Anti-Human IL-17F (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17F protein. A BLAST analysis was used to suggest cross-reactivity with IL-17F from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17F from other sources has not been determined.

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-IL-17F antibody, mouse anti-Interleukin-17F antibody, Cytokine ML-1, Interleukin-24

Immunohistochemistry of Mouse Anti-IL-17F antibody. Tissue: human colon tissue. Fixation: formalin-fixed, paraffin-embedded. Primary antibody: isotype control (top left), Mouse Anti-IL-17F antibody (full right and bottom left) at 5 ug/ml.

Western Blot of Mouse Anti-IL-17F antibody. Lane 1: human full length recombinant IL-17F protein. Lane 2: mouse full length recombinant IL-17F protein. Lane 3: rat full length recombinant IL-17F protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17F antibody at 0.5 ug/ml for overnight at 4°C.

Quick Database Links

UniProt

Q96PD4

RefSeq (Protein):NP_443104.1