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Catalog Number | orb1953416 |
---|---|
Category | Proteins |
Description | IL-15 & IL-15RA Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 34.46 kDa and the accession number is P48346-1 & Q60819-1. |
Tag | C-His |
Purity | SDS-PAGE: 98.1%; SEC-HPLC: 99.6% |
UniProt ID | Q60819, P48346 |
MW | 34.46 kDa (predicted); 50.02, 23.49, 21.78 and 19.76 kDa (reducing conditions) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | Mouse |
Biological Activity | IL-15 & IL-15RA Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 34.46 kDa and the accession number is P48346-1 & Q60819-1. |
Expression Region | A DNA sequence encoding the mouse IL15 (P48346-1)(Met1-Ser162) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse IL15RA (Q60819-1) (Met1-Lys205) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL15 & IL15RA heterodimer was purified. Predicted N terminal: Asn 49 & Gly 33 |
Storage | -20°C |
Note | For research use only |