IL-10 antibody

• Catalog Number: orb1089968
• Category: Antibodies
• Description: Rabbit monoclonal antibody to IL-10
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 9D7
• Tested applications: ELISA, IF, IHC, IP
• Reactivity: Human
• Isotype: IgG
• Immunogen: Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-10
• UniProt ID: P22301
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Human cytokine synthesis inhibitory factor;GVHDS;
• Note: For research use only
• Application notes: 9D7 has been used in ELISA experiments in order to specifically detect IL-10 and show higher circulating levels of IL-10 in patients with acute EBV-induced mononucleosis (Taga 1995). Further, 9D7 has been used to immunoprecipitate IL-10 from the supernatants of melanoma cells in order to study the production of IL-10 by melanoma cells (Qiyuan Chen 1994). A fab fragment derived from 9D7 has been used to study the noncompetitive antibody neutralisation of IL-10 by x-ray crystallography (Josephson 2002).
• Expiration Date: 12 months from date of receipt.