IKZF1 Antibody

• Catalog Number: orb1247470
• Category: Antibodies
• Description: IKZF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-STNSDDVRDEKVK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant G6PD captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IKZF1
• UniProt ID: P11413
• NCBI: NP_000393.4, NP_001035810.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: G6PD, glucose-6-phosphate dehydrogenase, G6PD1, gl
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant G6PD captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1247470 (0.03 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247470 (5 ug/ml) as the reporter antibody.

orb1247470 (2.5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247470 (2.5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.