IKK alpha/beta (phospho-S176/177) antibody

• Catalog Number: orb304723
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IKK alpha/beta (phospho-S176/177)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S176/177 of human IKK alpha/beta protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CHUK; IKBKB
• Entrez: 1147, 16150, 84351, 3551
• UniProt ID: O14920, O88351, Q9QY78, O15111, Q60680
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CHUK antibody, anti IKKA antibody, anti TCF16
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IKK alpha/beta (Phospho-S176/177) expression in HeLa LPS-treated (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 84; 86 kD; Observed band size: 85; 87 kD)

Immunohistochemical analysis of IKK alpha/beta (Phospho-S176/177) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.