IKK alpha Rabbit mAb [KD Validated] Antibody

SKU: orb1951502

Description

Plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB 1:5000~1:20000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human IKK alpha
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CHUK; I-kappa-B kinase 1; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK-A; IKK-a kinase; IKK-alpha; IKK1; IKKA;

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse kidney, using IKK alpha Antibody.

Western blot analysis of IKK alpha expression in Daudi cell lysate.

Quick Database Links

UniProt

O15111