IKBKE Antibody

• Catalog Number: orb1239458
• Category: Antibodies
• Description: IKBKE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-IKK epsilon antibody (orb1239458) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon. The immunogen is located within the last 50 amino acids of IKK epsilon.
• Concentration: 1 mg/ml
• Dilution range: WB: 1-2 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 80kDObserved: 80 kD
• Target: IKBKE
• UniProt ID: Q14164
• NCBI: AF241789
• Storage: IKK epsilon antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Induction Validation in Mouse Cell Line. Loading: 15 µg of Raw264.7 cell lysates per lane. Antibodies: IKK epsilon orb1239458 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Cells were treated with LPS (0.3 µg/mL) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Jurkat Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of IKK epsilon in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody (orb1239458) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of IKK epsilon in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with orb1239458 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).