IKB alpha/NFKBIA Antibody

• Catalog Number: orb234337
• Category: Antibodies
• Description: IKB alpha/NFKBIA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112). Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35609 MW
• UniProt ID: P25963
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alp
• Note: For research use only
• Application notes: WB: The detection limit for IKB alpha is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of IKB Alpha using anti-IKB Alpha antibody.Lane 1:human Jurkat cell;2:human MDA-MB-453 cell;3:human SW620 cell;4:human A549 cell.

WB analysis of IKB Alpha using anti-IKB Alpha antibody.Lane 1:rat brain tissue;2:rat lung tissue;3:rat heart tissue;4:rat skeletal muscle tissue;5:mouse lung tissue;6:mouse heart tissue.

IHC analysis of IKB alpha using anti-IKB alpha antibody.IKB alpha was detected in paraffin-embedded section of Mouse Intestine Tissue.

IHC analysis of IKB alpha using anti-IKB alpha antibody.IKB alpha was detected in paraffin-embedded section of Rat Intestine Tissue.

IHC analysis of IKB alpha using anti-IKB alpha antibody.IKB alpha was detected in paraffin-embedded section of Human Mammary Cancer Tissue.