IGHG4 Antibody

• Catalog Number: orb1252169
• Category: Antibodies
• Description: IGHG4 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IGHG4/1345
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: A human recombinant protein corresponding to the Fc region was used as the immunogen for this IgG4 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the IgG4 antibody to be titered up or down for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IGHG4
• UniProt ID: P01861
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the IgG4 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil with IgG4 antibody (clone IGHG4/1345). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.