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Catalog Number | orb248390 |
---|---|
Category | Antibodies |
Description | This antibody recognizes a protein of 75kDa, identified as the gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ICO-97 |
Tested applications | ELISA, FACS, IF |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Purified human IgG heavy chain was used as the immunogen for this anti-IgG antibody. |
Dilution range | ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This anti-IgG antibody is available for research use only. |
Entrez | 3500 |
Storage | Store the anti-IgG antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-IgG antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
ELISA, WB | |
Rabbit | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, WB | |
Canine, Rat, Sheep | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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