IGFBP3 Antibody

• Catalog Number: orb1272657
• Category: Antibodies
• Description: IGFBP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat SCF. Rat SCF specific antibody was purified by affinity chromatography employing an immobilized Rat SCF matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect hIGF-BP3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. SandwichTo detect hIGF-BP3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-BP3 (38-153) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. Western Blot:To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: IGFBP3
• UniProt ID: P17936
• NCBI: P17936
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: IBP3, BP-53, IBP3, Insulin-like growth factor-bind
• Note: For research use only
• Application notes: ELISA:Indirect:To detect hIGF-BP3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. SandwichTo detect hIGF-BP3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-BP3 (38-153) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. Western Blot:To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hIGF-BP3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP3 (orb1272742) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.

To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5 ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.