IGF1R / IGF1 Receptor Antibody (aa1264-1367, clone JBW902)

• Catalog Number: orb1537912
• Category: Antibodies
• Description: IGF1R / IGF1 Receptor Antibody (aa1264-1367, clone JBW902)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: JBW902
• Tested applications: IHC, IHC-P, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG2a
• Immunogen: GST fusion protein corresponding to residues 1264-1367 of human IGF-1 Receptor.
• Concentration: 1 mg/ml
• Dilution range: IHC, IHC-P (5 µg/ml), IP, WB
• Conjugation: Unconjugated
• Target: IGF1R / IGF1 Receptor
• Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
• Buffer/Preservatives: 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide. 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
• Alternative names: IGF1R, CD221 antigen, CD221, IGF1 Receptor, IGFR,
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B2905 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2905 was determined to be 5 ug/ml. Western Blot: 0.1-2 ug/ml. Detects the beta chain of IGF1R in RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells. C4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2905. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug/ml immunoprecipitates IGF1R from 1 mg of RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells.
• Expiration Date: 12 months from date of receipt.