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IGF1R Antibody / Insulin like growth factor 1 receptor

Catalog Number: orb2637279

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb2637279
CategoryAntibodies
DescriptionReceptor tyrosine kinases (RTKs) are transmembrane molecular scaffolds that influence cellular processes including the cell cycle, cell migration, cell metabolism, cell survival, proliferation and differentiation. Insulin-like growth factor-I receptor (IGF-IR) is an RTK that stimulates growth in many different cell types, blocks apoptosis, acts as an intermediate of many growth hormone responses and may stimulate the growth of some types of cancer. The IGF-IR cognate ligand Insulin-like growth factor-I (IGF-I) promotes association of IGF-IR with Shc, GRB2 and Sos 1, which initiates Ras and ERK kinase cascades, thereby modifying transcription factor activity, such as activation of the Elk transcription factors. The modular phosphotyrosine binding (PTB) domains of Insulin receptor substrate (IRS)-1 and -2 can associate with active IGF-IR and initiate phosphatidylinositol 3-kinase-dependent downstream signals. The human IGF-IR gene maps to chromosome 15q26.3 and encodes a 1,376 amino acid precursor protein that cleaves into subunits. The human IGF-IIR gene maps to chromosome 6q26 and encodes a 2,491 amino acid transmembrane protein.
Species/HostMouse
ClonalityMonoclonal
Clone NumberIGF1R/4667
Tested applicationsIHC-P
ReactivityHuman
IsotypeMouse IgG2c, kappa
ImmunogenA portion of amino acids 550-750 was used as the immunogen for the IGF1R antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml
PurityProtein A/G affinity
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis IGF1R antibody is available for research use only.
UniProt IDP08069
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the IGF1R antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
IGF1R Antibody / Insulin like growth factor 1 receptor

IHC staining of FFPE human prostate carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IGF1R Antibody / Insulin like growth factor 1 receptor

IHC staining of FFPE human breast carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IGF1R Antibody / Insulin like growth factor 1 receptor

IHC staining of FFPE human colon carcinoma tissue with IGF1R antibody (clone IGF1R/4667). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IGF1R Antibody / Insulin like growth factor 1 receptor

SDS-PAGE analysis of purified, BSA-free IGF1R antibody (clone IGF1R/4667) as confirmation of integrity and purity.

IGF1R Antibody / Insulin like growth factor 1 receptor

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using IGF1R antibody (clone IGF1R/4667). These results demonstrate the foremost specificity of the IGF1R/4667 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.