IGF1 R (phospho-Y1165/Y1166) antibody

• Catalog Number: orb214085
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IGF1R
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1165/Y1166 of human IGF1 Receptor protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: IGF1R
• Entrez: 25718, 16001, 3480
• UniProt ID: P24062, P08069, Q60751
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Insulin-like growth factor 1 receptor antibod
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IGF1 Receptor (Phospho-Y1165/Y1166) expression in MCF7 (A), MCF7-insulin-15min (B), A2780 (C) whole cell lysates. (Predicted band size: 154 kD; Observed band size: 95; 200 kD)

Immunohistochemical analysis of IGF1 Receptor (Phospho-Y1165/Y1166) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.