Igf1 Antibody

• Catalog Number: orb1272501
• Category: Antibodies
• Description: Igf1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Mouse
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine IGF-I.
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of murine IGF-I (200 ng/mL), a concentration of 12-14 μg/mL of this antibody is required. ELISA:To detect murine IGF-I by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine IGF-I. Sandwich:To detect mIGF-I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IGF-Ias a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.Western Blot:To detect murine IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine IGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: Igf1
• UniProt ID: P05017
• NCBI: P05017
• Storage: IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Igf-1, Igf-I, C730016P09Rik, Igf-1, Insulin-like g
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect mIGF-I by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IGF-I (orb1272500) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.

To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.