IgE antibody (Biotin)

• Catalog Number: orb623580
• Category: Antibodies
• Description: Mouse monoclonal antibody against IgE conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: BE5
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified human IgE.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-12 µg/ml
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: IgE
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Immunoglobulin E antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-12 µg/ml
• Expiration Date: 12 months from date of receipt.

Separation of human IgE positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgE (BE5) Biotin antibody (concentration in sample 4 μg/ml) Streptavidin APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (BE5) Biotin antibody (concentration in sample 4 μg/ml) Streptavidin APC.