IgE Antibody (APC)

• Catalog Number: orb1678979
• Category: Antibodies
• Description: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
• Clonality: Monoclonal
• Clone Number: 4H10
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified human IgE.
• Concentration: 0.1 mg/ml
• Conjugation: APC
• Target: IgE
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Immunoglobulin E
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 0.5-3 µg/ml
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human leukocytes stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml) and anti-human CD45 (MEM-28) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human IgE positive CD45dim basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml).