IFT74 Antibody

• Catalog Number: orb1247666
• Category: Antibodies
• Description: IFT74 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KTIVDALHSTSGN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276). Recommended concentration: 0.2-0.6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IFT74
• UniProt ID: Q96LB3
• NCBI: NP_001092692.1, NP_079379.2, AAK77221.1, NP_001092693.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intrafla
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276). Recommended concentration: 0.2-0.6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247666 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247666 (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

A) orb1247666 (2.5 mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control.