Ifngr1 Antibody

• Catalog Number: orb1671750
• Category: Antibodies
• Description: Ifngr1 Antibody
• Clonality: Recombinant
• Clone Number: GR20
• Tested applications: Blocking, FC, NeA
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising rats with interferon gamma receptor.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Ifngr1
• UniProt ID: P15261
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD119, Interferon gamma receptor 1, IFN gamma rece
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-IFN-gamma receptor 1 antibody GR20. Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of GR20 (orb1671750 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-IFN-gamma receptor 1 (GR20). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ cover-slips stained with the chimeric r version of GR20 (orb1671750) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671750- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.