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Catalog Number | orb1272505 |
---|---|
Category | Antibodies |
Description | Ifng Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Rat |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rIFN-gamma (rat Interferon-gamma). |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rIFN-gamma (0.3 ng/mL), a concentration of 0.006 - 0.01 μg/mL of this antibody is required. ELISA:To detect rIFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rIFN-gamma. Sandwich:To detect Rat IFN-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IFN-γas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-γ.Western Blot:To detect rIFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Ifng |
UniProt ID | P01581 |
NCBI | P01581 |
Storage | IFN-gamma antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | IFNG2Interferon gammaIFN-gamma Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect Rat IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IFN-gamma (orb1272504) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.
To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ICC, IF, IHC-P, WB | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, ICC, IHC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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