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Catalog Number | orb1539009 |
---|---|
Category | Antibodies |
Description | IFN Gamma / Interferon Gamma Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | E.coli derived recombinant Human IFN-gamma. |
Dilution range | ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) |
Conjugation | Unconjugated |
Target | IFN Gamma / Interferon Gamma |
Storage | Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2 |
Alternative names | IFNG, IFG, Immune interferon, IFI, Interferon gamm Read more... |
Note | For research use only |
Application notes | Resuspension: Reconstitute in sterile water to 0.1-1 mg/ml. . Further information: Immunohistochemistry: LS-B7487 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IFN-gamma (10 ng/ml), a concentration of 0.13-0.20 ug/ml of this antibody is required. ELISA: To detect Human IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-gamma (LS-C104708) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-gamma. Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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