IFN gamma (F2E4) Mouse Monoclonal Antibody

SKU: orb10876

Description

IFN gamma (F2E4) Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: FC, ICC
• Dilution range: Flow-Cyt=1ug/Test, ICC/IF=1:50-200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: F2E4
• Immunogen: Recombinant Full length of human IFN gamma (24-161/166 (C-6x His-Tag)aa)
• Target: IFNG
• Molecular Weight: 17 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

IFNG; IFG; IFI; IFN gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon; hIFN-gamma.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IFN gamma (4E2) Polyclonal Antibody, Unconjugated (orb10876) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IFNG

UniProt

P01579