IFN-gamma Antibody

• Catalog Number: orb1806421
• Category: Antibodies
• Description: IFN-gamma Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Residues 23-166
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: IFN-gamma
• UniProt ID: P01579
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: immune interferon; IFG; interferon gamma; IFN-gamm
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human IFN-gamma by western blot. Samples: Whole cell lysate (50 µg) from NK-92 cells untreated (-) and treated (+) with 80nM TPA, 3µM Ionomycin, 300ng/mL Brefeldin A. Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human IFN-gamma by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human IFN-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NK-92 cells treated with 80nM TPA, 3µM Ionomycin, and 300ng/mL Brefeldin A. Antibodies: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421) used for IP at 6 µl/mg lysate.

Detection of human IFN-gamma by immunocytochemistry. Sample: FFPE section of NK-92 cells treated with TPA, Ionomycin, Brefeldin A (left) and mock treated NK-92 cells (right). Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421). Secondary: HRP-conjugated goat anti-rabbit IgG.