You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1806420 |
---|---|
Category | Antibodies |
Description | IFN-gamma Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | ICC, IHC, IP, WB |
Reactivity | Human |
Immunogen | Residues 23-166 |
Concentration | 1000 µg/ml |
Dilution range | WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | IFN-gamma |
UniProt ID | P01579 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
Alternative names | immune interferon; IFG; interferon gamma; IFN-gamm Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human IFN-gamma by western blot. Samples: Whole cell lysate (50 µg) from NK-92 cells untreated (-) and treated (+) with 80nM TPA, 3µM Ionomycin, 300ng/mL Brefeldin A. Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806420) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human IFN-gamma by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806420). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human IFN-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NK-92 cells treated with 80nM TPA, 3µM Ionomycin, and 300ng/mL Brefeldin A. Antibodies: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806420) used for IP at 6 µl/mg lysate.
Detection of human IFN-gamma by immunocytochemistry. Sample: FFPE section of NK-92 cells treated with TPA, Ionomycin, Brefeldin A (left) and mock treated NK-92 cells (right). Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806420). Secondary: HRP-conjugated goat anti-rabbit IgG.
ICC, IF, IHC-P, WB | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, ICC, IHC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating