IFN gamma antibody

• Catalog Number: orb154408
• Category: Antibodies
• Description: Mouse monoclonal antibody against IFN gamma
• Clonality: Monoclonal
• Clone Number: 4S.B3
• Tested applications: ELISA, FC, ICC, IHC-P, IP, RIA, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Interferon gamma derived from human leukocytes
• Concentration: 1 mg/ml
• Dilution range: ELISA: This antibody is being used as detection antibody in combination with capture antibody NIB42. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: IFN gamma
• Entrez: 3458
• UniProt ID: P01579
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-IFG antibody, anti-IFI antibody, anti-IFN gam
• Note: For research use only
• Application notes: ELISA: This antibody is being used as detection antibody in combination with capture antibody NIB42. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of human IFN gamma positive lymphocytes (red-filled) from IFN gamma negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).