IFN gamma antibody (Biotin)

• Catalog Number: orb179917
• Category: Antibodies
• Description: Mouse monoclonal antibody against IFN gamma conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: 4S.B3
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Interferon gamma derived from human leukocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution 1-4 μg/ml. Intracellular staining. ELISA: Detection antibody in combination with capture antibody NIB42.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: IFN gamma
• Entrez: 3458
• UniProt ID: P01579
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IFG antibody, anti-IFI antibody, anti-IFN gam
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution 1-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Cytokine IFN gamma (IFNƴ) sandwich ELISA (enzyme-linked immunosorbent assay) that utilizes antibody clone NIB42 as the protein capture antibody and antibody clone 4S.B3-Biotin as the detection antibody. Recombinant IFNƴ was captured by NIB42 and detected by variable amounts of 4S.B3-Biotin. The 4S.B3-Biotin conjugate was visualized by Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of cytokine and to the amount of the detection antibody.

Flow cytometry multicolor intracellular staining pattern of human lymphocytes (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1.7 μg/ml, Streptavidin APC) and anti-human CD3 (UCHT1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human IFN-gamma positive CD3 positive lymphocytes (red-filled) from IFN-gamma negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1.7 μg/ml, Streptavidin APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1.7 μg/ml, Streptavidin APC).