IFI16 Antibody

• Catalog Number: orb1672345
• Category: Antibodies
• Description: IFI16 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Predicted Reactivity: Gorilla, Primate
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-IFI16 antibody (orb1672345) was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16. The immunogen is located within the last 50 amino acids of IFI16.
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 88kD Observed: 88 kD
• Target: IFI16
• UniProt ID: Q16666
• NCBI: Q16666.3
• Storage: IFI16 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IFI16 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IFI16 Antibody: Gamma-interferon-inducible protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

WB Validation in Human and Rat Cell Lines. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Testis. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of IFI16 in Human Molt4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with orb1672345 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of IFI16 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IFI16 in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IFI16 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.