IDH1 Antibody / Isocitrate Dehydrogenase (R132H mutant)

• Catalog Number: orb1151466
• Category: Antibodies
• Description: Rabbit monoclonal antibody to IDH1
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: IDH1/6806R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 281-414 was used as the immunogen for the IDH1 R132H antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This IDH1 R132H antibody is available for research use only.
• UniProt ID: O75874
• Storage: Aliquot the IDH1 R132H antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the IDH1 R132H antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of IDH1-R132H mutation protein in FFPE human prostate carcinoma tissue with IDH1-R132H antibody (clone IDH1/6806R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human glioblastoma tissue with IDH1-R132H antibody (clone IDH1/6806R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human cerebellum tissue with IDH1 R132H antibody (clone IDH1/6806R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.