ICOS-L Antibody / ICOS Ligand / B7RP-1

• Catalog Number: orb1824074
• Category: Antibodies
• Description: This mAb recognizes a protein of 36kDa, identified as ICOS-L, also called B7RP-1 or B7-related protein 1. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF Alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ICOSL/7700
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant fragment of human ICOSLG/B7RP-1 protein (within amino acids 1-300) was used as the immunogen for the B7RP-1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This B7RP-1 antibody is available for research use only.
• UniProt ID: O75144
• Storage: Aliquot the B7RP-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carcinoma tissue with B7RP-1 antibody (clone ICOSL/7700). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with B7RP-1 antibody (clone ICOSL/7700). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.