ICOS Antibody

• Catalog Number: orb1263980
• Category: Antibodies
• Description: ICOS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ICOS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the C-terminal region of human ICOS.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: ICOS
• UniProt ID: Q9Y6W8
• NCBI: Q9Y6W8
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Inducible T-cell costimulator, Activation-inducibl
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human lymph with ICOS Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis in Jurkat cell line lysates (35 ug/lane).This demonstrates the ICOS antibody detected the ICOS protein (arrow).

Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.