ICLN Antibody

• Catalog Number: orb1522851
• Category: Antibodies
• Description: ICLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 187 and 237
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ICLN
• UniProt ID: P54105
• NCBI: NP_001284.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: CLNS1B; ICln; reticulocyte protein ICln; methyloso
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse ICLN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody orb1522851 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ICLN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody orb1522851 used for IP at 6 µg per reaction.

Detection of human ICLN by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. orb1522851) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse ICLN by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. orb1522851) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human ICLN (shaded) in A549 cells by flow cytometry.Antibody: Rabbit anti-ICLN polyclonal antibody (orb1522851) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse ICLN (shaded) in A20 cells by flow cytometry.Antibody: Rabbit anti-ICLN polyclonal antibody (orb1522851) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.