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Catalog Number | orb1252521 |
---|---|
Category | Antibodies |
Description | ICAM3 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 101-1D2 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2a, kappa |
Immunogen | Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the ICAM-3 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ICAM3 |
UniProt ID | P32942 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Intercellular adhesion molecule 3, ICAM-3, CDw50, Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the ICAM-3 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE Analysis of Purified, BSA-Free ICAM-3 Antibody (clone 101-1D2). Confirmation of Integrity and Purity of the Antibody.
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