ICAM1 Antibody

• Catalog Number: orb402187
• Category: Antibodies
• Description: ICAM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ICAM1 recombinant protein (Position: Q28-R268).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 90 kDa
• UniProt ID: P05362
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Intercellular adhesion molecule 1; ICAM-1; Major g
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-ICAM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ICAM1 using anti-ICAM1 antibody.Lane 1:rat spleen Tissue;2:rat thymus Tissue;3:rat RH35 Cell;4:mouse spleen Tissue;5:mouse thymus Tissue;6:mouse HEPA1-6 Cell;7:mouse heart Tissue.

WB analysis of ICAM1 using anti-ICAM1 antibody.Lane 1:human Raji cell; 2:human K562 cell; 3:human HepG2 cell.

IF analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in immunocytochemical section of A431 cells.

IHC analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in paraffin-embedded section of human mammary cancer tissue.