IBMX

SKU: orb1307659

Description

IBMX is a non-selective phosphodiesterase inhibitor that targets PDE3, PDE4, and PDE5, thereby elevating intracellular cAMP levels. It is widely used in research to study cAMP-mediated signaling pathways in cellular and tissue models, including investigations into smooth muscle relaxation, inflammation, and cardiac function.

Research Area

Metabolism Research

Key Properties

• CAS Number: 28822-58-4
• MW: 222.24
• Purity: 99.99%
• Formula: C₁₀H₁₄N₄O₂
• SMILES: CC(C)Cn1c2[nH]cnc2c(=O)n(C)c1=O
• Target: PDE
• Solubility: DMSO:250 mg/mL (1124.91 mM);Ethanol:22.24 mg/mL (100.07 mM);H2O:2.23 mg/mL (10.03 mM);10% DMSO+90% Saline:1.67 mg/mL (7.51 mM)

Bioactivity

• Target IC50:
  PDE3:6.5 μM|THIK-1:120 μM|PDE4:26.3 μM|PDE5:31.7 μM
• In Vivo:
  METHODS: To test the metabolic effects on mice, IBMX (1 mg/kg) was injected subcutaneously into mice twice daily for seven days. RESULTS: IBMX significantly increased blood glucose levels in mice (blood glucose, mg/dl, control=141, IBMX=210). METHODS: To test the metabolic effects on hyperglycemic mice, glucose (0.5 g/kg) and IBMX (1 mg/kg) were injected i.v. in femoral veins into Wistar rats. RESULTS: In hyperglycemic rats, IBMX lowered blood glucose, IBMX did not change plasma insulin levels, and IBMX decreased hepatic glycogen stores.
• In Vitro:
  METHODS: CCDs isolated from HK-fed rats were pretreated with IBMX (100 μM) for 20 min and examined the effect of ANG II or cGMP on channel activity. RESULTS: IBMX activated ROMK channels and prevented further channel activation by ANG II. METHODS: Primary cultures of guinea pig TSMC were assayed for the effect of KMUP-1 on cAMP and cGMP levels in the presence of IBMX (100 μM). RESULTS: IBMX and KMUP-1 significantly increased cAMP and cGMP levels. The effect of KMUP-1 alone on cAMP and cGMP levels was not significantly different from that in the presence of IBMX. METHODS: Mammalian cell CHO was treated with IBMX (10-1000 μM) and whole cell currents were measured using the membrane clamp technique. RESULTS: A steady-state dose-response curve for the effect of IBMX on THIK-1 currents could be fitted with a Hill coefficient of 1 and an IC50 of 120 μM.
• Cell Research:
  Intracellular cyclic GMP and cyclic AMP concentrations in guinea-pig TSMCs were assayed as previously described. In brief, cells were grown in 24-well plates 10^5 cells per well. At confluence, monolayer cells were washed with phosphate buffer solution (PBS) and then incubated with KMUP-1 (0.1–100 μM) in the presence of 100 μM IBMX for 20 min. Incubation was terminated by the addition of 10% trichloroacetic acid (TCA). Cell suspensions were sonicated and then centrifuged at 2500 × g for 15 min at 4°C. To remove TCA, the supernatants were extracted three times with 5 volumes of water-saturated diethyl ether. Then, the supernatants were lyophilized and the cyclic GMP or AMP of each sample was determined by using commercially available radioimmunoassay kits .
• Animal Research:
  Male mice (25-35 g), obtained from the animal house of Faculty of Medicine, were kept in controlled environmental conditions (temperature: 23±2 oC; light-dark cycle: 7 a.m. to 7 p.m.) and were divided randomly into groups of seven. All test compounds were dissolved in DMSO and diluted to desired concentration with less than 1% DMSO. For the experiment, the test compound (IBMX, milrinone, MCPIP, mc1, mc2, mc5 or mc6) or solvent (control) was injected subcutaneously to mice at 1 mg/kg dosage twice a day (8:00 a.m. and 8:00 p.m.) for 7 days. On day 8, animals were anesthetized with intraperitoneal injection of thiopental (80 mg/kg) and blood samples were obtained from their hearts and then the liver was dissected. Each sample was centrifuged for 5 min and its serum was separated .

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Isobutylmethylxanthine, inhibit, PDE4, PDE3, Methylisobutylxanthine, IBMX, Inhibitor, 3-Isobutyl-1-methylxanthine, 1-Methyl-3-Isobutylxanthine, PDE5, phosphodiesterase, Phosphodiesterase (PDE)

Compound Identifiers

PubChem CID

3758