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Catalog Number | orb1946671 |
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Category | Assays and Kits |
Description | Hyp(Hydroxyproline) ELISA Kit |
Range | 78.13-5000 ng/mL |
Target | Hyp |
Sensitivity | 26.3 ng/mL |
Alternative names | (2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hydroxyproline(Hyp) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hydroxyproline(Hyp). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxyproline(Hyp) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
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