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Catalog Number | orb1957899 |
---|---|
Category | Proteins |
Description | HVEM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 19 kDa and the accession number is A0A024R052. |
Tag | C-His |
Purity | 98.70% |
MW | 19 kDa (predicted); 33-38 kDa (reducing condition, due to glycosylation) |
UniProt ID | Q92956 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | HVEM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 19 kDa and the accession number is A0A024R052. |
Expression Region | A DNA sequence encoding the extracellular domain (Met1-Val202) of human TNFRSF14 (NP_003811.2) was expressed fused with a C-terminal polyhistidine tag. Predicted N terminal: Pro 37 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide.The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 52-140 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE | |
Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
> 90% as determined by SDS-PAGE | |
18.4 kDa |
98.00% | |
20.6 kDa (predicted); 39.5 kDa (reducing conditions) |